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Genes important for survival or reproduction inVarroa destructoridentified by RNAi

Overview of attention for article published in Insect Science, December 2017
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#3 of 438)
  • High Attention Score compared to outputs of the same age (95th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

Mentioned by

news
6 news outlets
twitter
11 tweeters

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
14 Mendeley
Title
Genes important for survival or reproduction inVarroa destructoridentified by RNAi
Published in
Insect Science, December 2017
DOI 10.1111/1744-7917.12513
Pubmed ID
Authors

Zachary Y. Huang, Guowu Bian, Zhiyong Xi, Xianbing Xie

Abstract

The Varroa mite, (Varroa destructor), is the worst threat to honey bee health worldwide. To explore the possibility of using RNAi to control this pest, we determined the effects of knocking down various genes on Varroa mite survival and reproduction. Double-stranded RNA (dsRNA) of six candidate genes (Da, Pros26S, RpL8, RpL11, RpP0 and RpS13) were synthesized and each injected into Varroa mites, then mite survival and reproduction were assessed. Injection of dsRNA for Da (Daughterless) and Pros26S (Proteasome 26S subunit ATPase) caused a significant reduction in mite survival, with 3.57% ± 1.94% and 30.03% ± 11.43% mites surviving at 72 h post injection (hpi), respectively. Control mites injected with GFP-dsRNA showed survival rates of 81.95% ± 5.03% and 82.36 ± 2.81%, respectively. Injections of dsRNA for four other genes (RpL8, RpL11, RpP0 and RpS13) did not affect survival significantly, enabling us to assess their effect on Varroa mite reproduction. The number of female offspring per mite was significantly reduced for mites injected with dsRNA of each of these 4 genes compared to their GFP dsRNA control. Knockdown of the target genes was verified by real time PCR for two genes important for reproduction (RpL8, RpL11) and one gene important for survival (Pros26S). In conclusion, through RNA interference, we have discovered two genes important for mite survival and four genes important for mite reproduction. These genes could be explored as possible targets for the control of Varroa destructor in the future. This article is protected by copyright. All rights reserved.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 5 36%
Student > Ph. D. Student 3 21%
Student > Master 3 21%
Researcher 1 7%
Professor > Associate Professor 1 7%
Other 1 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 9 64%
Veterinary Science and Veterinary Medicine 2 14%
Biochemistry, Genetics and Molecular Biology 2 14%
Unspecified 1 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2017.
All research outputs
#271,690
of 12,357,335 outputs
Outputs from Insect Science
#3
of 438 outputs
Outputs of similar age
#12,885
of 267,880 outputs
Outputs of similar age from Insect Science
#1
of 16 outputs
Altmetric has tracked 12,357,335 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 97th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 438 research outputs from this source. They receive a mean Attention Score of 2.7. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 267,880 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.