↓ Skip to main content

Wiley Online Library

Genes important for survival or reproduction in Varroa destructor identified by RNAi

Overview of attention for article published in Insect Science, December 2017
Altmetric Badge

About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#7 of 915)
  • High Attention Score compared to outputs of the same age (96th percentile)
  • High Attention Score compared to outputs of the same age and source (96th percentile)

Mentioned by

news
6 news outlets
blogs
1 blog
twitter
9 X users

Citations

dimensions_citation
25 Dimensions

Readers on

mendeley
44 Mendeley
Title
Genes important for survival or reproduction in Varroa destructor identified by RNAi
Published in
Insect Science, December 2017
DOI 10.1111/1744-7917.12513
Pubmed ID
Authors

Zachary Y. Huang, Guowu Bian, Zhiyong Xi, Xianbing Xie

Abstract

The Varroa mite, (Varroa destructor), is the worst threat to honey bee health worldwide. To explore the possibility of using RNAi to control this pest, we determined the effects of knocking down various genes on Varroa mite survival and reproduction. Double-stranded RNA (dsRNA) of six candidate genes (Da, Pros26S, RpL8, RpL11, RpP0 and RpS13) were synthesized and each injected into Varroa mites, then mite survival and reproduction were assessed. Injection of dsRNA for Da (Daughterless) and Pros26S (Proteasome 26S subunit ATPase) caused a significant reduction in mite survival, with 3.57% ± 1.94% and 30.03% ± 11.43% mites surviving at 72 h post injection (hpi), respectively. Control mites injected with GFP-dsRNA showed survival rates of 81.95% ± 5.03% and 82.36 ± 2.81%, respectively. Injections of dsRNA for four other genes (RpL8, RpL11, RpP0 and RpS13) did not affect survival significantly, enabling us to assess their effect on Varroa mite reproduction. The number of female offspring per mite was significantly reduced for mites injected with dsRNA of each of these 4 genes compared to their GFP dsRNA control. Knockdown of the target genes was verified by real time PCR for two genes important for reproduction (RpL8, RpL11) and one gene important for survival (Pros26S). In conclusion, through RNA interference, we have discovered two genes important for mite survival and four genes important for mite reproduction. These genes could be explored as possible targets for the control of Varroa destructor in the future. This article is protected by copyright. All rights reserved.

X Demographics

X Demographics

The data shown below were collected from the profiles of 9 X users who shared this research output. Click here to find out more about how the information was compiled.
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 44 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 16%
Student > Bachelor 6 14%
Researcher 5 11%
Student > Master 5 11%
Professor 2 5%
Other 4 9%
Unknown 15 34%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 36%
Biochemistry, Genetics and Molecular Biology 5 11%
Veterinary Science and Veterinary Medicine 2 5%
Unspecified 1 2%
Mathematics 1 2%
Other 4 9%
Unknown 15 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 54. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 December 2021.
All research outputs
#736,685
of 24,451,065 outputs
Outputs from Insect Science
#7
of 915 outputs
Outputs of similar age
#17,170
of 447,155 outputs
Outputs of similar age from Insect Science
#1
of 33 outputs
Altmetric has tracked 24,451,065 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 915 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 447,155 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 96% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 96% of its contemporaries.