| Title |
DNA barcoding and minibarcoding as a powerful tool for feather mite studies
|
|---|---|
| Published in |
Molecular Ecology Resources, February 2015
|
| DOI | 10.1111/1755-0998.12384 |
| Pubmed ID | |
| Authors |
Jorge Doña, Javier Diaz-Real, Sergey Mironov, Pilar Bazaga, David Serrano, Roger Jovani |
| Abstract |
Feather mites (Astigmata: Analgoidea, Pterolichoidea) are among the most abundantand commonly occurring bird ectosymbionts. Basic questions on the ecology and evolution of feather mites remain unanswered because feather mite species identification is often only possible for adult males and it is laborious even for specialised taxonomists, thus precluding large-scale identifications. Here, we tested DNA barcoding as a useful molecular tool to identify feather mites from passerine birds. 361 specimens of 72 species of feather mites from 68 species of European passerine birds from Russia and Spain were barcoded. The accuracy of barcoding and mini-barcoding was tested. Moreover, threshold choice (a controversial issue in barcoding studies) was also explored in a new way, by calculating through simulations the effect of sampling effort (in species number and species composition) on threshold calculations. We found one 200 bp mini-barcode region that showed the same accuracy as the full-length barcode (602 bp) and was surrounded by conserved regions potentially useful for group-specific degenerate primers. Species identification accuracy was perfect (100%) but decreased when singletons or species of the Proctophyllodes pinnatus group were included. In fact, barcoding confirmed previous taxonomic issues within the Proctophyllodes pinnatus group. Following an integrative taxonomy approach, we compared our barcode study with previous taxonomic knowledge on feather mites, discovering three new putative cryptic species and validating three previous morphologically different (but still undescribed) new species. This article is protected by copyright. All rights reserved. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 33% |
| Spain | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 3% |
| Portugal | 2 | 2% |
| New Zealand | 1 | 1% |
| Mexico | 1 | 1% |
| Unknown | 85 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 18% |
| Researcher | 17 | 18% |
| Student > Master | 11 | 12% |
| Student > Bachelor | 10 | 11% |
| Professor > Associate Professor | 7 | 8% |
| Other | 18 | 20% |
| Unknown | 12 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 45 | 49% |
| Biochemistry, Genetics and Molecular Biology | 14 | 15% |
| Environmental Science | 13 | 14% |
| Linguistics | 1 | 1% |
| Medicine and Dentistry | 1 | 1% |
| Other | 1 | 1% |
| Unknown | 17 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 June 2015.
All research outputs
#3,626,130
of 24,542,484 outputs
Outputs from Molecular Ecology Resources
#577
of 1,740 outputs
Outputs of similar age
#44,255
of 260,171 outputs
Outputs of similar age from Molecular Ecology Resources
#15
of 40 outputs
Altmetric has tracked 24,542,484 research outputs across all sources so far. Compared to these this one has done well and is in the 85th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
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We're also able to compare this research output to 40 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 62% of its contemporaries.