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A novel mammalian cell line development platform utilizing nanofluidics and optoelectro positioning technology

Overview of attention for article published in Biotechnology Progress, September 2018
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#1 of 2,443)
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (99th percentile)

Mentioned by

news
14 news outlets
twitter
4 X users
patent
2 patents
wikipedia
1 Wikipedia page

Citations

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39 Dimensions

Readers on

mendeley
112 Mendeley
Title
A novel mammalian cell line development platform utilizing nanofluidics and optoelectro positioning technology
Published in
Biotechnology Progress, September 2018
DOI 10.1002/btpr.2690
Pubmed ID
Authors

Kim Le, Christopher Tan, Shivani Gupta, Trupti Guhan, Hedieh Barkhordarian, Jonathan Lull, Jennitte Stevens, Trent Munro

Abstract

Generating a highly productive cell line is resource intensive and typically involves long timelines because of the need to screen large numbers of candidates in protein production studies. This has led to miniaturization and automation strategies to allow for reductions in resources and higher throughput. Current approaches rely on the use of standard cell culture vessels and bulky liquid handling equipment. New nanofludic technologies offer novel solutions to surpass these limits, further miniaturizing cell culture volumes (105 times smaller) by growing cells on custom nanofluidic chips. Berkeley Lights' OptoElectro Positioning technology projects light patterns to activate photoconductors that gently repel cells to manipulate single cells on nanofluidic culturing chips. Using a fully integrated technology platform (Beacon), common cell culture tasks can be programmed through software, allowing maintenance and analysis of thousands of cell lines in parallel on a single chip. Here, we describe the ability to perform key cell line development work on the Beacon platform. We demonstrate that commercial production Chinese hamster ovary cell lines can be isolated, cultured, screened, and exported at high efficiency. We compare this process head to head with a FACS-enabled microtiter plate-based workflow and demonstrate generation of comparable clonal cell lines with reduced resources.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 112 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 112 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 27 24%
Student > Ph. D. Student 24 21%
Student > Bachelor 9 8%
Other 8 7%
Student > Master 5 4%
Other 9 8%
Unknown 30 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 28 25%
Agricultural and Biological Sciences 19 17%
Engineering 11 10%
Chemical Engineering 4 4%
Chemistry 4 4%
Other 13 12%
Unknown 33 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 118. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 September 2023.
All research outputs
#336,127
of 24,535,155 outputs
Outputs from Biotechnology Progress
#1
of 2,443 outputs
Outputs of similar age
#7,411
of 346,427 outputs
Outputs of similar age from Biotechnology Progress
#1
of 26 outputs
Altmetric has tracked 24,535,155 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,443 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 346,427 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 26 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 99% of its contemporaries.