| Title |
Minimal Tags for Rapid Dual‐Color Live‐Cell Labeling and Super‐Resolution Microscopy
|
|---|---|
| Published in |
Angewandte Chemie. International Edition, January 2014
|
| DOI | 10.1002/anie.201309847 |
| Pubmed ID | |
| Authors |
Ivana Nikić, Tilman Plass, Oliver Schraidt, Jędrzej Szymański, John A. G. Briggs, Carsten Schultz, Edward A. Lemke |
| Abstract |
The growing demands of advanced fluorescence and super-resolution microscopy benefit from the development of small and highly photostable fluorescent probes. Techniques developed to expand the genetic code permit the residue-specific encoding of unnatural amino acids (UAAs) armed with novel clickable chemical handles into proteins in living cells. Here we present the design of new UAAs bearing strained alkene side chains that have improved biocompatibility and stability for the attachment of tetrazine-functionalized organic dyes by the inverse-electron-demand Diels-Alder cycloaddition (SPIEDAC). Furthermore, we fine-tuned the SPIEDAC click reaction to obtain an orthogonal variant for rapid protein labeling which we termed selectivity enhanced (se) SPIEDAC. seSPIEDAC and SPIEDAC were combined for the rapid labeling of live mammalian cells with two different fluorescent probes. We demonstrate the strength of our method by visualizing insulin receptors (IRs) and virus-like particles (VLPs) with dual-color super-resolution microscopy. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 308 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | <1% |
| Czechia | 2 | <1% |
| Netherlands | 1 | <1% |
| Sweden | 1 | <1% |
| Portugal | 1 | <1% |
| Denmark | 1 | <1% |
| Korea, Republic of | 1 | <1% |
| Japan | 1 | <1% |
| Unknown | 297 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 89 | 29% |
| Researcher | 58 | 19% |
| Student > Master | 31 | 10% |
| Student > Bachelor | 30 | 10% |
| Professor | 11 | 4% |
| Other | 36 | 12% |
| Unknown | 53 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 97 | 31% |
| Biochemistry, Genetics and Molecular Biology | 61 | 20% |
| Agricultural and Biological Sciences | 58 | 19% |
| Physics and Astronomy | 9 | 3% |
| Immunology and Microbiology | 5 | 2% |
| Other | 21 | 7% |
| Unknown | 57 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 October 2023.
All research outputs
#5,165,888
of 25,374,647 outputs
Outputs from Angewandte Chemie. International Edition
#16,471
of 49,993 outputs
Outputs of similar age
#57,853
of 322,946 outputs
Outputs of similar age from Angewandte Chemie. International Edition
#237
of 597 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. Compared to these this one has done well and is in the 79th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 49,993 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has gotten more attention than average, scoring higher than 66% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 322,946 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 82% of its contemporaries.
We're also able to compare this research output to 597 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.