| Title |
Alu element‐containing RNAs maintain nucleolar structure and function
|
|---|---|
| Published in |
EMBO Journal, October 2015
|
| DOI | 10.15252/embj.201591458 |
| Pubmed ID | |
| Authors |
Maïwen Caudron‐Herger, Teresa Pankert, Jeanette Seiler, Attila Németh, Renate Voit, Ingrid Grummt, Karsten Rippe |
| Abstract |
Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 20% |
| Unknown | 4 | 80% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Scientists | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 172 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Spain | 1 | <1% |
| United States | 1 | <1% |
| Denmark | 1 | <1% |
| Unknown | 168 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 43 | 25% |
| Student > Ph. D. Student | 40 | 23% |
| Student > Bachelor | 18 | 10% |
| Student > Master | 11 | 6% |
| Professor > Associate Professor | 10 | 6% |
| Other | 22 | 13% |
| Unknown | 28 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 69 | 40% |
| Agricultural and Biological Sciences | 52 | 30% |
| Medicine and Dentistry | 4 | 2% |
| Neuroscience | 3 | 2% |
| Engineering | 3 | 2% |
| Other | 8 | 5% |
| Unknown | 33 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 33. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 November 2015.
All research outputs
#1,033,314
of 22,830,751 outputs
Outputs from EMBO Journal
#335
of 12,239 outputs
Outputs of similar age
#16,646
of 279,229 outputs
Outputs of similar age from EMBO Journal
#13
of 49 outputs
Altmetric has tracked 22,830,751 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 12,239 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.8. This one has done particularly well, scoring higher than 97% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 279,229 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.