Title |
Alu element‐containing RNAs maintain nucleolar structure and function
|
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Published in |
EMBO Journal, October 2015
|
DOI | 10.15252/embj.201591458 |
Pubmed ID | |
Authors |
Maïwen Caudron‐Herger, Teresa Pankert, Jeanette Seiler, Attila Németh, Renate Voit, Ingrid Grummt, Karsten Rippe |
Abstract |
Non-coding RNAs play a key role in organizing the nucleus into functional subcompartments. By combining fluorescence microscopy and RNA deep-sequencing-based analysis, we found that RNA polymerase II transcripts originating from intronic Alu elements (aluRNAs) were enriched in the nucleolus. Antisense-oligo-mediated depletion of aluRNAs or drug-induced inhibition of RNA polymerase II activity disrupted nucleolar structure and impaired RNA polymerase I-dependent transcription of rRNA genes. In contrast, overexpression of a prototypic aluRNA sequence increased both nucleolus size and levels of pre-rRNA, suggesting a functional link between aluRNA, nucleolus integrity and pre-rRNA synthesis. Furthermore, we show that aluRNAs interact with nucleolin and target ectopic genomic loci to the nucleolus. Our study suggests an aluRNA-based mechanism that links RNA polymerase I and II activities and modulates nucleolar structure and rRNA production. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 20% |
Unknown | 4 | 80% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 60% |
Scientists | 2 | 40% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | <1% |
Spain | 1 | <1% |
United States | 1 | <1% |
Denmark | 1 | <1% |
Unknown | 168 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 43 | 25% |
Student > Ph. D. Student | 40 | 23% |
Student > Bachelor | 18 | 10% |
Student > Master | 11 | 6% |
Professor > Associate Professor | 10 | 6% |
Other | 22 | 13% |
Unknown | 28 | 16% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 69 | 40% |
Agricultural and Biological Sciences | 52 | 30% |
Medicine and Dentistry | 4 | 2% |
Neuroscience | 3 | 2% |
Engineering | 3 | 2% |
Other | 8 | 5% |
Unknown | 33 | 19% |